Tehran University of Medical Sciences Frontiers in Biomedical Technologies 2345-5837 2 2 2015 06 30 115 117 EN Aram Radnia Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Hossein Farahani Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran. Hoorvash C.Yousefzadeh Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Reza Ay Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, IranAND Department of Medical Physics and Biomedical Engineering, Tehran University of Medical Sciences, Tehran, Iran. 2015 11 24 2015 11 24 Purpose: In this report the design concept and experimental evaluation of the performance of HeliGuide have been illustrated.  Methods: 14C-UBT system designed for detecting Helicobacter pylori. In order to assess the capability of the system, results of 221 reference cards have been analyzed. Results: Results of the HeliGuide system are matched with the reference.  Conclusion: Results of the evaluation indicated that HeliGuide is adequate as a Urea Breath Test system for detecting helicobacter pylori. https://fbt.tums.ac.ir/index.php/fbt/article/view/53 https://fbt.tums.ac.ir/index.php/fbt/article/download/53/48