Tehran University of Medical Sciences Frontiers in Biomedical Technologies 2345-5837 11 1 2023 12 31 104 112 Zeinab Hormozi-Moghaddam Radiation Biology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran Manijhe Mokhtari-Dizaji Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Mohammad Ali Nilforoshzade Skin and Stem Cells Research Center, Tehran University of Medical Sciences, Tehran, Iran Mohsen Bakhshande Department of Radiology Technology, Allied Medical Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran Sona Zare Skin and Stem Cells Research Center, Tehran University of Medical Sciences, Tehran, Iran 2023 02 01 2023 02 24 Purpose: High-resolution ultrasound imaging is a non-invasive and objective appraisal. Ultrasound imaging accomplishes the target assessment and follow-up of radiation-induced skin injury. The study aimed to investigate the complete anatomical and structural alternations of acute wound healing in skin tissue radiation injury after cell therapy with high-frequency ultrasound imaging techniques. Materials and Methods: Female guinea pigs (250 g) were divided into 3 groups: (a) controls, consisting of non-treated guinea pigs; (b) radiation-treated; (c) radiation-treated receiving adipose-derived mesenchymal stem cells. Acute radiation-induced skin injury was induced by a single fraction of X-ray irradiation of 60Gy to a 3.0×3.0-cm area with a 1.3-cm bolus on 100-cm SSD in the abdominal skin tissue. Ultrasonic imaging of the depth and quality of healing in the skin tissue was performed by processing ultrasound images at 40-MHz and 75-MHz frequencies. Results: Skin thickness indicated a significant difference between the treatment and control groups on Day 10 after 60 Gy irradiation (P<0.05). The highest skin thickness was observed in the irradiated group, and the lowest skin thickness was found in the stem cell treatment group. Conclusion: Evaluation of skin thickness, wound depth, and scar formation is important for the proper assessment and management of wound healing in stem cell therapy of radiation-induced skin damage. High-resolution ultrasound at 40- and 75-MHz frequencies is a major non-invasive method providing unprecedented insight into determining the characterization of the skin, particularly in the context of wound healing. https://fbt.tums.ac.ir/index.php/fbt/article/view/642 https://fbt.tums.ac.ir/index.php/fbt/article/download/642/384