Tehran University of Medical Sciences Frontiers in Biomedical Technologies 2345-5837 12 4 2025 10 01 703 711 Mohammed Abdulameer Alobeidi Master Student, Department of Aesthetic and Restorative dentistry, College of Dentistry, University of Baghdad. Raghad Abdulrazzaq Alhashimi Department of Aesthetic and Restorative dentistry, College of Dentistry, University of Baghdad, Iraq 2023 07 17 2023 09 12 Purpose: This in-vitro study was conducted to illustrate the influence of Traditional Endodontic Access Cavity (TEAC) and Conservative Endodontic Access Cavity (CEAC) procedures on the capacity of AF F One (Fanta Dental Materials Co., Shanghai, China) and AF Blue R3 (Fanta Dental Materials Co., Shanghai, China), a continuous rotation and reciprocation endodontic rotary file systems respectively, to shape the root canals of maxillary premolar teeth without transportation or centering deviation. Materials and Methods: Randomly, forty maxillary premolar teeth were categorized into four groups. Group 1 was accessed and instrumented conventionally with AF F One, Group 2 was accessed and instrumented conventionally with AF Blue R3, Group 3 was accessed and instrumented conservatively with AF F One, and Group 4 was accessed and instrumented conservatively with AF Blue R3. With the use of Cone-Beam Computed Tomography (CBCT) imaging (Planmeca ProMax, Helsinki, ‎Finland), before and after instrumentation pictures of the root canals were captured for the purpose of calculating the transportation and centering ability. Results: After checking the normality of data distribution, which was accomplished using the Shapiro-Wilk test, an independent sample t-test was performed to compare the data of various groups with a 95% level of confidence. Equivalent in terms of transportation and centering capacity, the two distinct endodontic instruments have comparable shaping capacities. No statistically significant difference (P > 0.05) was seen among the endodontic access cavity approaches for the two file systems investigated. Conclusion: Compared to TEAC preparation, CEAC preparation has no effect on the capacity of the endodontic files to shape the root canal system. https://fbt.tums.ac.ir/index.php/fbt/article/view/758 https://fbt.tums.ac.ir/index.php/fbt/article/download/758/532