Tehran University of Medical Sciences Frontiers in Biomedical Technologies 2345-5837 12 1 2025 01 01 161 165 Saade Abdalkareem Jasim edical Laboratory Techniques Department, Al-maarif University College, Anbar, Iraq Bashar Mudhaffar Abdullah Clinical Laboratory Technology Department, Al-Rafidain University College, Baghdad, Iraq Patricio Yánez-Moretta Professor Escuela de Ciencias Biológicas e Ingeniería, Universidad de Investigación de Tecnología Experimental Yachay, Urcuquí, Ecuador Wesam R. Kadhum Department of Pharmacy, Kut University College, Kut 52001, Wasit, Iraq Yasser Fakri Mustafa Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul-41001, Iraq Enas R. Alwaily Microbiology Research Group, College of Pharmacy, Al-Ayen University, Thi-Qar, Iraq Munther Abosoda College of Pharmacy, The Islamic University of Babylon, Iraq Ali Alsalamy College of Technical Engineering, Imam Ja’afar Al‐Sadiq University, Al‐Muthanna 66002, Iraq Sada Ghalib Al-Musawi College of Pharmacy, National University of Science and Technology, Dhi Qar, Iraq Alaa A. Omran Department of Engineering, AL-Nisour University College, Baghdad, Iraq 2023 11 29 2023 12 10 Purpose: Plantago Major (PM) is widely used for the treatment of different diseases due to several active compounds. Previous studies demonstrated the treatment effect of this plant on lung cancer cell lines. Here, we introduced a patient having lung cancer proved by Computed Tomography (CT) and pathological findings. The treatment effect of PM was assessed and presented based on CT and laboratory examinations for this patient as a first human case study. Materials and Methods: A 64-year-old woman, with gastrointestinal bleeding as well as high Erythrocyte Sedimentation Rate (ESR) and C-Reactive Protein (CRP) levels, was examined by the chest CT scan for suspicious lung cancer. Pathological findings confirmed the non-small cell lung cancer (adenocarcinoma, stage IIA). The patient consumed 150 ml of PM seeds extract (3-4 times daily) orally for about 4 months. Follow-up CT and laboratory examinations were performed after the treatment period to assess the effect of PM. Results: The volume of the tumor was reduced by about 62% (based on CT imaging findings) after the treatment with PM. In addition, the laboratory examinations illustrate that ESR and CRP levels reduced remarkably (from 97 mm/h to 24 mm/h for ESR; from 36.8 mg/L to 1.2 mg/L for CRP) after the treatment. Conclusion: Based on our human study, PM, as a natural compound with antioxidant, anti-inflammatory, and antibiotic characteristics, could have an anti-proliferative effect on non-small cell lung cancer. However, more follow-up examinations on big sample sizes are needed to assess the treatment effect of PM. https://fbt.tums.ac.ir/index.php/fbt/article/view/894 https://fbt.tums.ac.ir/index.php/fbt/article/download/894/376