Tehran University of Medical Sciences Frontiers in Biomedical Technologies 2345-5837 12 3 2025 07 01 541 546 Hind Ibrahim Khaleel Department of Chemistry, Collage of Science, Baghdad University, Department of Chemistry, Collage of Science, Baghdad University, Ibn Sina University of medical and pharmaceutical Sciences. Nada Qasim Kadhim Department of Chemistry, Collage of Science, Baghdad University, Baghdad, Iraq Ameer Qasim Abdalrida College of Medicine, Ibn Sina University of Medical and Pharmaceutical Sciences, Baghdad, Iraq 2024 02 28 2024 03 18 Purpose: The goal of biomedical researchers is to overcome harmful bacteria' resistance to antibiotics by developing new active chemicals quickly, cheaply, easily, and environmentally. Materials and Methods: In this instance, iron nanoparticles (Fe-NPs) were created using a green method by mixing an equal volume of Zingiber officinale aqueous extract with a 100 ml solution of ferrous chloride tetrahydrate (FeCl2.4H2O (0.4 M)). The distinctive dark brown color shift of the mixture indicated the production of Fe-NPs. Results: This suggests that the nanocomposite was created and the reaction occurred. FT-IR, TEM, and UV-Vis spectroscopy were utilized to analyze phytosynthesized Fe-NPs. Overall, the phytosynthesized Fe-NPs show activities that enable their use in various biomedical and biotechnological applications. Additionally, the antimicrobial effect was investigated against Gram-negative bacteria (Escherichia coli). Conclusion: The antibacterial activity of E. coli was determined, and the highest zone of inhibition was observed at the concentration of 100 μg/mL. https://fbt.tums.ac.ir/index.php/fbt/article/view/952 https://fbt.tums.ac.ir/index.php/fbt/article/download/952/442