Tehran University of Medical Sciences Frontiers in Biomedical Technologies 2345-5837 12 3 2024 07 11 468 478 Navid Kheradmand Department of Health Physics, Graduate School of Health Sciences, İstanbul Medipol University, İstanbul, Türkiye Omid Talaee Department of Nuclear Engineering, Faculty of Mechanical Engineering, University of Shiraz, Iran Fatemeh Tabatabaee Department of Medical Physics, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Vahideh Alvandi Department of Physics, Faculty of Sciences, Razi University, Kermanshah, Iran Emad Khoshdel Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Parizad Ghadimi Department of Medical Physics and Radiology, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran Mustafa ÇAĞLAR Department of Health Physics, Graduate School of Health Sciences, İstanbul Medipol University, İstanbul, Türkiye. 2024 03 26 2024 05 19 Purpose: The incorporation of Nanoparticles (NPs) in Computed Tomography (CT) imaging significantly enhances the contrast, clarity, and sensitivity of CT scans, leading to a substantial improvement in the accuracy and reliability of diagnostic information obtained from the images. The objective of the current research was to investigate the application of gold (Au) NPs in enhancing the imaging capabilities of Breast Cancer (BC) cells. Materials and Methods: Au NPs were synthesized by loading Trastuzumab (TZ) on PEGylated Au NPs. Firstly, Au NPs were produced and coated with PEG-SH to form PEG-Au NPs. Next, TZ was coupled with OPSS-PEG-SVA to enable its attachment to the PEG-Au NPs. The resulting NPs were characterized for their structure, size, and morphology using standard analytical techniques. To assess the potential of the developed NPs for CT scan imaging of BC cells, SKBr-3 cells were treated with Au NPs and TZ-PEG-Au NPs. Additionally, the cytotoxicity of the NPs was evaluated with the MTT technique. Results: The SEM and TEM analyses revealed that the synthesized NPs exhibited a spherical shape and displayed a relatively uniform size distribution (approximately 45 nm). The results showed that the developed Au NPs have acceptable biocompatibility and superior X-ray attenuation properties compared to a commonly used contrast agent. Conclusion: Based on our results, it can be concluded that the proposed TZ- Polyethylene Glycol-Au NPs are suitable for CT imaging of BC cells. https://fbt.tums.ac.ir/index.php/fbt/article/view/980 https://fbt.tums.ac.ir/index.php/fbt/article/download/980/417