Ultrasmall Superparamagnetic Iron Oxide Nanoparticles as Emerging Contrast Agents for Enhanced T2-Weighted Magnetic Resonance Imaging
-
Mahboobeh Mehrabifard
,
Solmaz Derakhshan
,
Roqaie Kalantari
,
Amirhossein Rashnoodi
,
Saadat Ebrahimiyan
,
Sahar Mohammadjani
,
Omid Talaee
,
Navid Kheradmand
Abstract
Purpose: Integrating magnetic Nanoparticles (NPs) into contrast-enhanced Magnetic Resonance (MR) imaging can significantly improve the resolution and sensitivity of the resulting images, leading to enhanced accuracy and reliability in diagnostic information. The present study aimed to investigate the use of targeted trastuzumab-labeled iron oxide (TZ-PEG-Fe3O4) NPs to enhance imaging capabilities for the detection and characterization of Breast Cancer (BC) cells.
Materials and Methods: The NPs were synthesized by loading Fe3O4NPs with the monoclonal antibody TZ. Initially, Fe3O4 NPs were produced and subsequently coated with Polyethylene Glycol (PEG) to form PEG- Fe3O4 NPs. The TZ antibody was then conjugated to the PEG- Fe3O4 NPs, resulting in TZ-PEG-Fe3O4 NPs. The resulting NPs were characterized using standard analytical techniques, including UV-Vis spectroscopy, FTIR, SEM, TEM, VSM, and assessments of colloidal stability.
Results: Analyses indicated that the targeted TZ-PEG-Fe3O4 NPs exhibited a spherical morphology and a relatively uniform size distribution, with an average diameter of approximately 60 nm. These results confirmed the successful synthesis and controlled fabrication of the Fe3O4 NPs, which is crucial for developing effective Contrast Agents (CAs) for medical imaging applications. Additionally, the study confirmed the biocompatibility and magnetic properties of the synthesized TZ-PEG-Fe3O4 NPs.
Conclusion: The findings suggest that the developed targeted TZ-PEG-Fe3O4 NPs have significant potential as effective CAs for MR imaging of BC cells.
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16- Gholibegloo E Mansouri H, Mortezazadeh T, Yazdi MH, Ashouri F, Malekzadeh R, Najafi A, Foroumadi A, Khoobi M. A biocompatible theranostic nanoplatform based on magnetic gadolinium-chelated polycyclodextrin: in vitro and in vivo studies. Carbohydrate Polymers. 2021 Feb 15;254:117262.
17- Phinikaridou A Sandiford L, Protti A, Meszaros LK, Cui X, Yan Y, Frodsham G, Williamson PA, Gaddum N, Botnar RM, Blower PJ. Bisphosphonate-anchored PEGylation and radiolabeling of superparamagnetic iron oxide: long-circulating nanoparticles for in vivo multimodal (T1 MRI-SPECT) imaging. Acs Nano. 2013 Jan 22;7(1):500-12.
18- Han B Wei Y, Hu X, Lin Y, Wang X, Deng X. Synthesis of Fe3O4 nanoparticles and their magnetic properties. Procedia Engineering. 2012 Jan 1;27:632-7.
19- Ghorbani M Abdollahi BB, Hamishehkar H, Malekzadeh R, Farajollahi A. Synthesis and characterization of actively HER-2 Targeted Fe3O4@ Au nanoparticles for molecular radiosensitization of breast cancer. BioImpacts: BI. 2023;13(1):17.
20- Yang K Zi Y, He J, Wu Z, Liu J, Zhang W. Strategies to enhance drug delivery to solid tumors by harnessing the EPR effects and alternative targeting mechanisms. Advanced Drug Delivery Reviews. 2022 Sep 1;188:114449.
21- Ballarino L Dousset V, Delalande C, Coussemacq M, Canioni P, Petry KG, Caillé JM. Comparison of ultrasmall particles of iron oxide (USPIO)-enhanced T2-weighted, conventional T2-weighted, and gadolinium-enhanced T1-weighted MR images in rats with experimental autoimmune encephalomyelitis. American journal of neuroradiology. 1999 Feb 1;20(2):223-7.
22- Singh LH Pati SS, Guimarães EM, Mantilla J, Coaquira JA, Oliveira AC, Sharma VK, Garg VK. Magnetic chitosan-functionalized Fe3O4@ Au nanoparticles: Synthesis and characterization. Journal of Alloys and Compounds. 2016 Nov 5;684:68-74.
23- Wang K Gao Y, Zhang J, Duan X, Sun Q, Men K. Multifunctional nanoparticle for cancer therapy. MedComm. 2023 Feb;4(1):e187.
24- Kim HS Kim J, Lee N, Kim T, Kim H, Yu T, Song IC, Moon WK, Hyeon T. Multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance and fluorescence imaging and for drug delivery. Angewandte Chemie-International Edition. 2008 Oct 20;47(44):8438-41.
25- Bronstein LM Mahmoud WE, Al-Hazmi F, Al-Noaiser F, Al-Ghamdi AA. Development of Fe/Fe3O4 core–shell nanocubes as a promising magnetic resonance imaging contrast agent. Langmuir. 2013 Oct 22;29(42):13095-101.
26- Sanavio B Sousa F, Saccani A, Tang Y, Zucca I, Carney TM, Mastropietro A, Jacob Silva PH, Carney RP, Schenk K, Omrani AO. Superparamagnetic nanoparticles as high efficiency magnetic resonance imaging T2 contrast agent. Bioconjugate chemistry. 2017 Jan 18;28(1):161-70. .
27- Khademi F Parvaneh S, Abdi G, Alizadeh A, Mostafaie A. Efficient conjugation of anti-HBsAg antibody to modified core-shell magnetic nanoparticles (Fe3O4@ SiO2/NH2). BioImpacts: BI. 2021;11(4):237.
28- Rayamajhi S Marasini R, Moreno-Sanchez A, Aryal S. Iron (iii) chelated paramagnetic polymeric nanoparticle formulation as a next-generation T 1-weighted MRI contrast agent. RSC advances. 2021;11(51):32216-26.
2- Tarighatnia A Mohaghegh S, Omidi Y, Barar J, Aghanejad A, Adibkia K. Multifunctional magnetic nanoparticles for MRI-guided co-delivery of erlotinib and L-asparaginase to ovarian cancer. Journal of Microencapsulation. 2022 May 19;39(4):394-408.
3- Tarighatnia A Jamshidi N, Ghaziyani MF, Sajadian F, Olad-Ghaffari M, Nader ND. Folic acid-conjugated Fe-Au-based nanoparticles for dual detection of breast cancer cells by magnetic resonance imaging and computed tomography. Frontiers in Biomedical Technologies. 2023 Dec 26.
4- Khoei S Amraee A, Mahdavi SR, Tohidkia MR, Tarighatnia A, Darvish L, Hosseini Teshnizi S, Aghanejad A. Ultrasmall iron oxide nanoparticles and gadolinium-based contrast agents in magnetic resonance imaging: a systematic review and meta-analysis. Clinical and Translational Imaging. 2023 Feb;11(1):83-93.
5- Babaye Abdollahi B Malekzadeh R, Ghorbani M, Pirayesh Islamian J, Mortezazadeh T. Trastuzumab conjugated PEG–Fe3O4@ Au nanoparticle as an MRI biocompatible nano-contrast agent. International Journal of Polymeric Materials and Polymeric Biomaterials. 2023 Jul 3;72(10):759-70.
6- Ghorbani M Malekzadeh R, Faghani P, Abdollahi BB, Mortezazadeh T, Farhood B. Fabrication of targeted gold nanoparticle as potential contrast agent in molecular CT imaging. Journal of Radiation Research and Applied Sciences. 2023 Mar 1;16(1):100490.
7- Malekzadeh R Ziyaee S, Ghorbani M, Nasiri Motlagh B, Asghariazar V, Mortezazadeh T. Preparation of MnO2@ poly-(DMAEMA-co-IA)-conjugated methotrexate nano-complex for MRI and radiotherapy of breast cancer application. Magnetic Resonance Materials in Physics, Biology and Medicine. 2023 Oct;36(5):779-95.
8- Ayyami Y Yektamanesh M, Ghorbani M, Dastgir M, Malekzadeh R, Mortezazadeh T. Characterization of multifunctional β-cyclodextrin-coated Bi2O3 nanoparticles conjugated with curcumin for CT imaging-guided synergetic chemo-radiotherapy in breast cancer. International Journal of Pharmaceutics. 2024 Jun 25;659:124264.
9- Mortezazadeh T Alipour B, Abdulsahib WK, Arzhang A, Malekzadeh R, Farhood B. A systematic review of multimodal application of quantum dots in breast cancer diagnosis: Effective parameters, status and future perspectives. Journal of Drug Delivery Science and Technology. 2023 Sep 1;86:104682.
10- Xuan S J Wang YX, Port M, Idee JM. Recent advances in superparamagnetic iron oxide nanoparticles for cellular imaging and targeted therapy research. Current pharmaceutical design. 2013 Nov 1;19(37):6575-93.
11- Park J Yu MK, Jon S. Targeting strategies for multifunctional nanoparticles in cancer imaging and therapy. Theranostics. 2012;2(1):3.
12- Vega MA Nieto C, Martin del Valle EM. Trastuzumab: more than a guide in HER2-positive cancer nanomedicine. Nanomaterials. 2020 Aug 26;10(9):1674.
13- Araujo F Nunes R, Tavares J, Sarmento B, das Neves J. Surface modification with polyethylene glycol enhances colorectal distribution and retention of nanoparticles. European Journal of Pharmaceutics and Biopharmaceutics. 2018 Sep 1;130:200-6.
14- Development and Applications of the Silica-Based Contrast Agents in Magnetic Resonance Imaging. Current Nanomedicine (Formerly: Recent Patents on Nanomedicine). 2018 Apr 1;8(1):3-27. Chowdhury MA. Design.
15- Gao L Yang M, Liu K, Luo C, Wang Y, Yu L, Peng H, Zhang W. Characterization of Fe3O4/SiO2/Gd2O (CO3) 2 core/shell/shell nanoparticles as T1 and T2 dual mode MRI contrast agent. Talanta. 2015 Jan 1;131:661-5.
16- Gholibegloo E Mansouri H, Mortezazadeh T, Yazdi MH, Ashouri F, Malekzadeh R, Najafi A, Foroumadi A, Khoobi M. A biocompatible theranostic nanoplatform based on magnetic gadolinium-chelated polycyclodextrin: in vitro and in vivo studies. Carbohydrate Polymers. 2021 Feb 15;254:117262.
17- Phinikaridou A Sandiford L, Protti A, Meszaros LK, Cui X, Yan Y, Frodsham G, Williamson PA, Gaddum N, Botnar RM, Blower PJ. Bisphosphonate-anchored PEGylation and radiolabeling of superparamagnetic iron oxide: long-circulating nanoparticles for in vivo multimodal (T1 MRI-SPECT) imaging. Acs Nano. 2013 Jan 22;7(1):500-12.
18- Han B Wei Y, Hu X, Lin Y, Wang X, Deng X. Synthesis of Fe3O4 nanoparticles and their magnetic properties. Procedia Engineering. 2012 Jan 1;27:632-7.
19- Ghorbani M Abdollahi BB, Hamishehkar H, Malekzadeh R, Farajollahi A. Synthesis and characterization of actively HER-2 Targeted Fe3O4@ Au nanoparticles for molecular radiosensitization of breast cancer. BioImpacts: BI. 2023;13(1):17.
20- Yang K Zi Y, He J, Wu Z, Liu J, Zhang W. Strategies to enhance drug delivery to solid tumors by harnessing the EPR effects and alternative targeting mechanisms. Advanced Drug Delivery Reviews. 2022 Sep 1;188:114449.
21- Ballarino L Dousset V, Delalande C, Coussemacq M, Canioni P, Petry KG, Caillé JM. Comparison of ultrasmall particles of iron oxide (USPIO)-enhanced T2-weighted, conventional T2-weighted, and gadolinium-enhanced T1-weighted MR images in rats with experimental autoimmune encephalomyelitis. American journal of neuroradiology. 1999 Feb 1;20(2):223-7.
22- Singh LH Pati SS, Guimarães EM, Mantilla J, Coaquira JA, Oliveira AC, Sharma VK, Garg VK. Magnetic chitosan-functionalized Fe3O4@ Au nanoparticles: Synthesis and characterization. Journal of Alloys and Compounds. 2016 Nov 5;684:68-74.
23- Wang K Gao Y, Zhang J, Duan X, Sun Q, Men K. Multifunctional nanoparticle for cancer therapy. MedComm. 2023 Feb;4(1):e187.
24- Kim HS Kim J, Lee N, Kim T, Kim H, Yu T, Song IC, Moon WK, Hyeon T. Multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance and fluorescence imaging and for drug delivery. Angewandte Chemie-International Edition. 2008 Oct 20;47(44):8438-41.
25- Bronstein LM Mahmoud WE, Al-Hazmi F, Al-Noaiser F, Al-Ghamdi AA. Development of Fe/Fe3O4 core–shell nanocubes as a promising magnetic resonance imaging contrast agent. Langmuir. 2013 Oct 22;29(42):13095-101.
26- Sanavio B Sousa F, Saccani A, Tang Y, Zucca I, Carney TM, Mastropietro A, Jacob Silva PH, Carney RP, Schenk K, Omrani AO. Superparamagnetic nanoparticles as high efficiency magnetic resonance imaging T2 contrast agent. Bioconjugate chemistry. 2017 Jan 18;28(1):161-70. .
27- Khademi F Parvaneh S, Abdi G, Alizadeh A, Mostafaie A. Efficient conjugation of anti-HBsAg antibody to modified core-shell magnetic nanoparticles (Fe3O4@ SiO2/NH2). BioImpacts: BI. 2021;11(4):237.
28- Rayamajhi S Marasini R, Moreno-Sanchez A, Aryal S. Iron (iii) chelated paramagnetic polymeric nanoparticle formulation as a next-generation T 1-weighted MRI contrast agent. RSC advances. 2021;11(51):32216-26.
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How to Cite
1.
Mehrabifard M, Derakhshan S, Kalantari R, Rashnoodi A, Ebrahimiyan S, Mohammadjani S, Talaee O, Kheradmand N. Ultrasmall Superparamagnetic Iron Oxide Nanoparticles as Emerging Contrast Agents for Enhanced T2-Weighted Magnetic Resonance Imaging. Frontiers Biomed Technol. 2024;.
