Investigation of Atmospheric Pressure Plasma Jet Effects on the Treatment of Glioblastoma Using PET Imaging
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Abstract
Purpose: Cold Atmospheric Plasma (CAP) has a wide application in medicine that has many biological effects on bacterial, fungal, yeast, and mammalian cells, particularly cancer cells. In this study, the in-vivo anti-tumor effect of an Atmospheric Pressure Jet Plasma (APJP) source is investigated.
Materials and Methods: The Glioblastoma Multiforme (GBM) tumoric cells have been implanted in the rat brains. Then, cancerian cells are treated by direct CAP and the Temozolomide (TMZ) drug. Positron Emission Tomography (PET) imaging method is used for evaluating the tumor regions.
Results: The PET imaging results showed that the GBM cell has a significant reduction in direct plasma treatment and TMZ drug just. This reduction can be clearly observed just five days after treatment. The calculated Brain to the Background Ratio (BBR) shows that the cancer cells in the plasma method are halved after five days. This amount is comparable to the TMZ method that makes two times decreasing in the cancer cells.
Conclusion: The plasma method can be used as an effective method in treating and removing the GBM cells. Also, CAP has fewer side effects than chemical methods.
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3- P. Attri, N. Kumar, J.H Park,. D.K. Yadav, S.Choi, H.S.Uhm,; I.T Kim.; E.H. Choi, W.Lee, "Influence of reactive species on the modification of biomolecules generated from the soft plasma.", Sci. Rep., 5, 08221, (2015).
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7- E. Robert, M. Vandamme.; L. Brullé.; S. Lerondel; A. le Pape, V. Sarron, D. Riès, T. Darny, S. Dozias, G. Collet, "Perspectives of endoscopic plasma applications." Clin. Plasma Med., 1, 8–16, (2013).
8- Z. Chen, L. Lin, X. Cheng, E. Gjika, M. Keidar, "Effects of cold atmospheric plasma generated in deionized water in cell cancer therapy.", Plasma Process. Polym., 13, 1151–1156, (2016).
9- S. Kalghatgi, G. Friedman, A. Fridman, A.M. Clyne, "Endothelial cell proliferation is enhanced by low dose non-thermal plasma through fibroblast growth factor-2 release.", Ann. Biomed. Eng., 38, 748–757, (2010).
10- H.M. Joh, S.J. Kim, T. Chung, S. Leem, "Reactive oxygen species-related plasma effects on the apoptosis of human bladder cancer cells in atmospheric pressure pulsed plasma jets.", Appl. Phys. Lett., 101, 053703, (2012).
11- D.N. Cagney, A.M. Martin, P.J. Catalano, A.J. Redig, N.U.Lin, E.Q. Lee, P.Y Wen, I.F. Bi W.L Dunn, S.E Weiss, et al, "Incidence and prognosis of patients with brain metastases at diagnosis of systemic malignancy: A population-based study.", Neuro-Oncology, 19, 1511–1521, (2017).
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13- C. Tendero, C. Tixier, P. Tristant, Desmaison, J.; Leprince, P. "Atmospheric pressure plasmas: A review. Spectrochim.", Acta Part. BAt. Spectrosc., 61, 2–30, (2006).
14- D. Yan, J.H. Sherman, M. Keidar, "Cold atmospheric plasma, a novel promising anti-cancer treatment modality.", Oncotarget, 8, 15977–15995, (2016).
15- M. Kordt, I. Trautmann, Ch. Schlie , T. Lindner, J. Stenzel, A. Schildt, , Boeckmann, L., Bekeschus, S., Kurth, J., Krause B. J., Vollmar, Grambow E., "Multimodal Imaging Techniques to Evaluate the Anticancer Effect of Cold Atmospheric Pressure Plasma.", Cancers, 13, 2483, (2021). https://doi.org/10.3390/cancers13102483.
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19- Y. Park, H. J.Kim, J. Y. Lee, S. H Jeong, E. Y. Kwon, J. Y. Joo, "Anti-inflammatory effect of non-thermal atmospheric pressure plasma for periodontitis treatment: in vitro pilot study.", Journal of Dental Rehabilitation and Applied Science, , 37(2), 88-94, (2021).
20- S. Bekeschus, J.Moritz, I. Helfrich, L. Boeckmann, K. D. Weltmann, S. Emmert, T. Von Woedtke, "Ex vivo exposure of human melanoma tissue to cold physical plasma elicits apoptosis and modulates inflammation.", Applied Sciences, , 10(6), 1971, (2020).
21- H. R. Metelmann, C. Seebauer, V. Miller, A. Fridman, G. Bauer, D. B. Graves, T. Von Woedtke, "Clinical experience with cold plasma in the treatment of locally advanced head and neck cancer.", Clinical Plasma Medicine, 9, 6-13, (2018).
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| Issue | Vol 9 No 3 (2022) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/fbt.v9i3.9649 | |
| Keywords | ||
| Cold Atmospheric Plasma Positron Emission Tomography Imaging Glioblastoma Multiforme Anticancer Effects Atmospheric Pressure Jet Plasma | ||
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How to Cite
1.
Bakhtiari Ramezani M, Yahaghi E, Nohekhan M. Investigation of Atmospheric Pressure Plasma Jet Effects on the Treatment of Glioblastoma Using PET Imaging. Frontiers Biomed Technol. 2022;9(3):224-230.
