Original Article

Electromagnetic Field (EMF) Effect on Bone Marrow Stem Cells (BMSCs) Differentiation and Nucleoli AgNOR


Purpose: Numerous studies have described the effect of Electromagnetic Fields (EMFs) in the promotion of Bone Marrow Stem Cell (BMSC) differentiation. We aimed to investigate the influence of frequency (10 and 100 Hz) and different pulse shapes (sine, rectangular, and triangular) of EMF on rats' BMSCs.

Materials and Methods: The BMSCs in 6 groups were exposed to EMF for 1 h/ 7 days. The BMSCs viability was estimated by the MTT test. The cresyl violet labeled the Nissl bodies, and the silver nitrate staining was done to evaluate the BMSCs nucleoli Ag Nucleolar Organizer Regions (AgNORs).

Results: The MTT test verified that EMF and pulse shape did not affect cell viability. In Nissl bodies staining most of the large neurons were related to the rectangular 10 Hz EMF group. The majority of the differentiated BMSCs were astrocytes, microglia, and oligodendrocyte in the triangular 100 Hz EMF group. Although the silver nitrate staining confirmed the effect of 10 Hz EMF, pulse shape alteration did not affect AgNOR parameters. In conclusion, we presented a low-magnetic flux density EMF (400 ┬ÁT) to assess the responses of BMSCs nuclei.

Conclusion: The findings showed that BMSCs differentiation was frequency-dependent. Further investigations are recommended for recognizing the function of EMF on BMSCs.

IssueVol 10 No 2 (2023) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/fbt.v10i2.12217
Electromagnetic Field Bone Marrow Stem Cell Cell Viability Differentiation Ag Nucleolar Organizer Regions

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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Asadian N, Jadidi T, Jadidi M. Electromagnetic Field (EMF) Effect on Bone Marrow Stem Cells (BMSCs) Differentiation and Nucleoli AgNOR. Frontiers Biomed Technol. 2022;10(2):132-139.