Comparative Study on Biosynthesizing Selenium Nanoparticles by Gum Arabic and Poly Anionic Cellulose to Prevent Radiation-Induced Death in Chinese Hamster Ovary (CHO) Cells
-
Mojgan Hasanzade
,
Mohammad-Taghi Bahreyni-Toossi
,
Majid Darroudi
,
Sara Khademi
,
Fereshteh Vaziri-Nezamdoost
,
Hosein Azimian
Abstract
Purpose: In premenopausal women, abdominopelvic radiotherapy may have a direct and profound effect on ovarian function. Stabilized selenium Nanoparticles (NPs) with some natural materials have been demonstrated to have high antioxidant activity and reduce radiation damage as a radioprotector. This study was done to compare the ability for the biosynthesis of selenium NPs by Gum Arabic (Se-GA) and Polyanionic Cellulose (Se-PAC) in the protection of Chinese Hamster Ovary (CHO) cells against radiation damage.
Materials and Methods: First, Selenium Nanoparticles (SeNPs) were synthesized in the presence of GA and PAC. Then, CHO cells were cultured in-vitro and were randomly divided into six groups in different concentrations of Se-GA and Se-PAC to measure the biocompatibility of NPs. Finally, cells were treated with NPs and radiation (6MV, 2Gy), and the percentage of cell survival was determined by MTT assay. Both NPs with an average size of 20-30 nm and an absorption absorbance peak at about 300 nm using Ultraviolet-Visible (UV–Vis) spectroscopy.
Results: According to the parametric t-test analysis, Se-GA nanoparticles with a concentration higher than 0.4 ppm significantly increased the radioprotective effect on CHO cells compared to the control group (P<0.05). However, Se-PAC showed no significant increase in radioprotection in contrast to the control group (P>0.05).
Conclusion: Se-GA nanoparticles have antioxidant properties, and the radiation protection properties of Se-GA nanoparticles are significantly higher than control. Consequently, Se-GA nanoparticles showed promising results and may be able to play the role of a radioprotector.
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13- Yanhui Zhou et al., "Green synthesis of Se/Ru alloy nanoparticles using gallic acid and evaluation of theiranti-invasive effects in HeLa cells." Colloids and Surfaces B: Biointerfaces, Vol. 144pp. 118-24, (2016).
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15- Mahin Velayati, Hassan Hassani, Zahra Sabouri, Asma Mostafapour, and Majid Darroudi, "Biosynthesis of Se-Nanorods using Gum Arabic (GA) and investigation of their photocatalytic and cytotoxicity effects." Inorganic Chemistry Communications, Vol. 128p. 108589, 2021/06/01/(2021).
16- Mahin Velayati, Hassan Hassani, and Majid Darroudi, "Green synthesis of Se-Nanorods using Poly Anionic Cellulose (PAC) and examination of their photocatalytic and cytotoxicity effects." Inorganic Chemistry Communications, Vol. 133p. 108935, 2021/11/01/(2021).
17- SK Torres et al., "Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity." Journal of Nanoparticle Research, Vol. 14 (No. 11), p. 1236, (2012).
18- Bo Huang, Jinsong Zhang, Jingwu Hou, and Chang Chen, "Free radical scavenging efficiency of Nano-Se in vitro." Free Radical Biology and Medicine, Vol. 35 (No. 7), pp. 805-13, (2003).
19- Huali Wang, Jinsong Zhang, and Hanqing Yu, "Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: Comparison with selenomethionine in mice." Free Radical Biology and Medicine, Vol. 42 (No. 10), pp. 1524-33, 2007/05/15/ (2007).
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21- Badreldin H Ali, Amal Ziada, and Gerald Blunden, "Biological effects of gum arabic: a review of some recent research." Food and chemical Toxicology, Vol. 47 (No. 1), pp. 1-8, (2009).
22- EM Hussein, "Modulatory role of arabic gum in gamma rays-induced damages in rats." J. Rad. Res. Appl. Sci, Vol. 1 (No. 2), pp. 427-41 ,(2008).
23- Heba A Mohamed, Ahmed S Nada, Neamat Hanafi, Hala F Zaki, and Sanaa A Kenawy, "The reno protective effect of gum arabic in gamma-irradiated and cisplatin treated rats." International Journal of Scientific and Research Publications, Vol. 5 (No. 6), (2015).
24- JS Djajadisastra, Pony Purnamasari, and ANUNG Pujiyanto, "Antioxidant activity of gold nanoparticles using gum arabic as a stabilizing agent." Int J Pharm Pharm Sci, Vol. 6 (No. 7), pp. 462-5, (2014).
25- Huiling Kong, Jixin Yang, Yifeng Zhang, Yapeng Fang, Katsuyoshi Nishinari, and Glyn O Phillips, "Synthesis and antioxidant properties of gum arabic-stabilized selenium nanoparticles." International journal of biological macromolecules, Vol. 65pp. 155-62, (2014).
26- A Hassani, SA Hussain, N Abdullah, S Kamarudin, and R Rozita, "Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum arabic-stabilised magnesium orotate nanoparticles." International Food Research Journal, Vol. 27 (No. 1), (2020).
27- A Hassani et al., "Formulation and antioxidant properties of curcumin gum Arabic nanoparticles for delivery to cancer cells." in IOP Conference Series: Materials Science and Engineering, (2020), Vol. 991 (No. 1): IOP Publishing, p. 012101.
28- Karl Buch, Tanja Peters, Thomas Nawroth, Markus Sänger, Heinz Schmidberger, and Peter Langguth, "Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay-A comparative study." Radiation oncology, Vol. 7 (No. 1), pp. 1-6,(2012).
29- Zohre Rezaee, Ali Yadollahpour, and Vahid Bayati, "Single intense microsecond electric pulse induces radiosensitization to ionizing radiation: effects of time intervals between electric pulse and Ionizing Irradiation." Frontiers in oncology, Vol. 8p. 418, (2018).
30- Zohre Rezaee, Ali Yadollahpour, Vahid Bayati, and Fereshteh Negad Dehbashi, "Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study." International Journal of Nanomedicine, Vol. 12p. 1431, (2017).
31- Sergey Gudkov et al., "Production and application of selenium nanoparticles to prevent ionizing radiation-induced oxidative stress." in IOP Conference Series: Earth and Environmental Science, (2019), Vol. 390 (No. 1): IOP Publishing, p. 012031.
32- Hanan A Fahmy, Afrag Sh Abd El Azim, and Ola A Gharib, "Protective effects of omega-3 fatty acids and/or nano-selenium on cisplatin and ionizing radiation induced liver toxicity in rats." Indian Journal of Pharmaceutical Education and Research, Vol. 50 (No. 4), pp. 649-56, (2016).
33- Hejia Zhang, Qingjia Sun, Lingling Tong, Yanru Hao, and Tianyu Yu, "Synergistic combination of PEGylated selenium nanoparticles and X-ray-induced radiotherapy for enhanced anticancer effect in human lung carcinoma." Biomedicine & Pharmacotherapy, Vol. 107pp. 1135-41, (2018).
34- Bo Yu, Ting Liu, Yanxin Du, Zuandi Luo, Wenjie Zheng, and Tianfeng Chen, "X-ray-responsive selenium nanoparticles for enhanced cancer chemo-radiotherapy." Colloids and Surfaces B: Biointerfaces, Vol. 139pp. 180-89, (2016).
35- Yahui Yang et al., "Functionalized selenium nanosystem as radiation sensitizer of 125I seeds for precise cancer therapy." ACS applied materials & interfaces, Vol. 9 (No. 31), pp. 25857-69, (2017).
2- Casey M Cosgrove and Ritu Salani, "Ovarian effects of radiation and cytotoxic chemotherapy damage." Best Practice & Research Clinical Obstetrics & Gynaecology, Vol. 55pp. 37-48, (2019).
3- C Haie-Meder et al., "Radiotherapy after ovarian transposition: ovarian function and fertility preservation." International Journal of Radiation Oncology* Biology* Physics, Vol. 25 (No. 3), pp. 419-24, (1993).
4- Qinqi Wang et al., "Radioprotective Effect of Flavonoids on Ionizing Radiation-Induced Brain Damage." Molecules, Vol. 25 (No. 23), p. 5719, (2020).
5- Qiongge Hu, Yunxiang Zhou, Shijie Wu, Wei Wu, Yongchuan Deng, and Anwen Shao, "Molecular hydrogen: A potential radioprotective agent." Biomedicine & Pharmacotherapy, Vol. 130p. 110589, (2020).
6- H Bagheri et al., "Radioprotective Effects of Zinc and Selenium on Mice Spermatogenesis." Journal of Biomedical Physics & Engineering, Vol. 10 (No. 6), p. 707 ,(2020).
7- Richard J Lobb, Gregory M Jacobson, Ray T Cursons, and Michael B Jameson, "The interaction of selenium with chemotherapy and radiation on normal and malignant human mononuclear blood cells." International journal of molecular sciences, Vol. 19 (No. 10), p. 3167, (2018).
8- JF Weiss, V Srinivasan, KS Kumar, and MR Landauer, "Radioprotection by metals: selenium." Advances in Space Research, Vol. 12 (No. 2-3), pp. 223-31, (1992).
9- 1006" poster Influence of selenium on the radiosensitivity of glioma cells." Radiotherapy and Oncology, Vol. 73, pp. S423-S24, 2004/10/01/ (2004).
10- Bagher Farhood et al., "Selenium as an adjuvant for modification of radiation response." Journal of cellular biochemistry, Vol. 120 (No. 11), pp. 18559-71 , (2019).
11- Masoumeh Karami, Siamak Asri-Rezaei, Banafshe Dormanesh, and Ali Nazarizadeh, "Comparative study of radioprotective effects of selenium nanoparticles and sodium selenite in irradiation-induced nephropathy of mice model." International journal of radiation biology, Vol. 94 (No. 1), pp. 17-27 ,(2018).
12- Feng Chen, Xiao Hong Zhang, Xiao Dan Hu, Pei Dang Liu, and Hai Qian Zhang, "The effects of combined selenium nanoparticles and radiation therapy on breast cancer cells in vitro." Artificial cells, nanomedicine, and biotechnology, Vol. 46 (No. 5), pp. 937-48 , (2018).
13- Yanhui Zhou et al., "Green synthesis of Se/Ru alloy nanoparticles using gallic acid and evaluation of theiranti-invasive effects in HeLa cells." Colloids and Surfaces B: Biointerfaces, Vol. 144pp. 118-24, (2016).
14- S. K. Torres et al., "Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity." Journal of Nanoparticle Research, Vol. 14 (No. 11), p. 1236, 2012/10/20 (2012).
15- Mahin Velayati, Hassan Hassani, Zahra Sabouri, Asma Mostafapour, and Majid Darroudi, "Biosynthesis of Se-Nanorods using Gum Arabic (GA) and investigation of their photocatalytic and cytotoxicity effects." Inorganic Chemistry Communications, Vol. 128p. 108589, 2021/06/01/(2021).
16- Mahin Velayati, Hassan Hassani, and Majid Darroudi, "Green synthesis of Se-Nanorods using Poly Anionic Cellulose (PAC) and examination of their photocatalytic and cytotoxicity effects." Inorganic Chemistry Communications, Vol. 133p. 108935, 2021/11/01/(2021).
17- SK Torres et al., "Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity." Journal of Nanoparticle Research, Vol. 14 (No. 11), p. 1236, (2012).
18- Bo Huang, Jinsong Zhang, Jingwu Hou, and Chang Chen, "Free radical scavenging efficiency of Nano-Se in vitro." Free Radical Biology and Medicine, Vol. 35 (No. 7), pp. 805-13, (2003).
19- Huali Wang, Jinsong Zhang, and Hanqing Yu, "Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: Comparison with selenomethionine in mice." Free Radical Biology and Medicine, Vol. 42 (No. 10), pp. 1524-33, 2007/05/15/ (2007).
20- SK Torres et al., "Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity." Journal of Nanoparticle Research, Vol. 14 (No. 11), pp. 1-9, (2012).
21- Badreldin H Ali, Amal Ziada, and Gerald Blunden, "Biological effects of gum arabic: a review of some recent research." Food and chemical Toxicology, Vol. 47 (No. 1), pp. 1-8, (2009).
22- EM Hussein, "Modulatory role of arabic gum in gamma rays-induced damages in rats." J. Rad. Res. Appl. Sci, Vol. 1 (No. 2), pp. 427-41 ,(2008).
23- Heba A Mohamed, Ahmed S Nada, Neamat Hanafi, Hala F Zaki, and Sanaa A Kenawy, "The reno protective effect of gum arabic in gamma-irradiated and cisplatin treated rats." International Journal of Scientific and Research Publications, Vol. 5 (No. 6), (2015).
24- JS Djajadisastra, Pony Purnamasari, and ANUNG Pujiyanto, "Antioxidant activity of gold nanoparticles using gum arabic as a stabilizing agent." Int J Pharm Pharm Sci, Vol. 6 (No. 7), pp. 462-5, (2014).
25- Huiling Kong, Jixin Yang, Yifeng Zhang, Yapeng Fang, Katsuyoshi Nishinari, and Glyn O Phillips, "Synthesis and antioxidant properties of gum arabic-stabilized selenium nanoparticles." International journal of biological macromolecules, Vol. 65pp. 155-62, (2014).
26- A Hassani, SA Hussain, N Abdullah, S Kamarudin, and R Rozita, "Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum arabic-stabilised magnesium orotate nanoparticles." International Food Research Journal, Vol. 27 (No. 1), (2020).
27- A Hassani et al., "Formulation and antioxidant properties of curcumin gum Arabic nanoparticles for delivery to cancer cells." in IOP Conference Series: Materials Science and Engineering, (2020), Vol. 991 (No. 1): IOP Publishing, p. 012101.
28- Karl Buch, Tanja Peters, Thomas Nawroth, Markus Sänger, Heinz Schmidberger, and Peter Langguth, "Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay-A comparative study." Radiation oncology, Vol. 7 (No. 1), pp. 1-6,(2012).
29- Zohre Rezaee, Ali Yadollahpour, and Vahid Bayati, "Single intense microsecond electric pulse induces radiosensitization to ionizing radiation: effects of time intervals between electric pulse and Ionizing Irradiation." Frontiers in oncology, Vol. 8p. 418, (2018).
30- Zohre Rezaee, Ali Yadollahpour, Vahid Bayati, and Fereshteh Negad Dehbashi, "Gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in HT-29 tumor cells: an in vitro study." International Journal of Nanomedicine, Vol. 12p. 1431, (2017).
31- Sergey Gudkov et al., "Production and application of selenium nanoparticles to prevent ionizing radiation-induced oxidative stress." in IOP Conference Series: Earth and Environmental Science, (2019), Vol. 390 (No. 1): IOP Publishing, p. 012031.
32- Hanan A Fahmy, Afrag Sh Abd El Azim, and Ola A Gharib, "Protective effects of omega-3 fatty acids and/or nano-selenium on cisplatin and ionizing radiation induced liver toxicity in rats." Indian Journal of Pharmaceutical Education and Research, Vol. 50 (No. 4), pp. 649-56, (2016).
33- Hejia Zhang, Qingjia Sun, Lingling Tong, Yanru Hao, and Tianyu Yu, "Synergistic combination of PEGylated selenium nanoparticles and X-ray-induced radiotherapy for enhanced anticancer effect in human lung carcinoma." Biomedicine & Pharmacotherapy, Vol. 107pp. 1135-41, (2018).
34- Bo Yu, Ting Liu, Yanxin Du, Zuandi Luo, Wenjie Zheng, and Tianfeng Chen, "X-ray-responsive selenium nanoparticles for enhanced cancer chemo-radiotherapy." Colloids and Surfaces B: Biointerfaces, Vol. 139pp. 180-89, (2016).
35- Yahui Yang et al., "Functionalized selenium nanosystem as radiation sensitizer of 125I seeds for precise cancer therapy." ACS applied materials & interfaces, Vol. 9 (No. 31), pp. 25857-69, (2017).
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| Issue | Vol 11 No 2 (2024) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/fbt.v11i2.15340 | |
| Keywords | ||
| Radiotherapy Radiation Protection Gum Arabic Nanoparticles Ovary | ||
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How to Cite
1.
Hasanzade M, Bahreyni-Toossi M-T, Darroudi M, Khademi S, Vaziri-Nezamdoost F, Azimian H. Comparative Study on Biosynthesizing Selenium Nanoparticles by Gum Arabic and Poly Anionic Cellulose to Prevent Radiation-Induced Death in Chinese Hamster Ovary (CHO) Cells. Frontiers Biomed Technol. 2024;11(2):239-246.
