Folic Acid-Conjugated Fe-Au-Based Nanoparticles for Dual Detection of Breast Cancer Cells by Magnetic Resonance Imaging and Computed Tomography
-
Nasim Jamshidi
,
Ali Tarighatnia
,
Mona Fazel Ghaziyani
,
Fakhrossadat Sajadian
,
Maryam Olad ghaffari
,
Nader D Nader
Abstract
Purpose: We synthesized folic acid-conjugated Fe3O4/Au-pralidoxime chloride Nanoparticles (Fe2O3/Au@PAM NPs) for use as dual-modal contrast agents for Magnetic Resonance Imaging (MRI) and Computed Tomography (CT) in the diagnosis of breast cancer.
Materials and Methods: Fe2O3/Au@PAM NPs labeled or not to folic acid were synthesized and analyzed by dynamic light scattering, transmission electron microscopy, and vibrating sample magnetometry. The ability of these NPs to create image contrast was also investigated in silico and in vitro (in MCF-7 breast cancer cells and A549 lung cancer cells) with CT and MRI.
Results: Dynamic light scattering and transmission electron microscopy revealed that the Fe2O3/Au@PAM NPs were nearly spherical. The average diameter of Fe2O3/Au NPs increased from 11.6 nm to 98 nm after folic acid conjugation. The saturation magnetization values of Fe2O3/Au@PAM NPs with and without folic acid conjugation were 25.56 and 32.6 emu/g, respectively. Conjugation of folic acid to NPs greatly improved their uptake by cancer cells. The additional coating of NPs with FA reduced the T2 relaxation time and signal intensity for MRI. Folic acid-labeled MCF-7 cells had a radiodensity measurement of 208 Hunsfield Units (HU) compared to 95 HU for A549 cells. For breast cancer cells, NPs labeled with folic acid significantly improved the X-ray absorption coefficient as a sign of active cellular uptake compared to NPs without labeling.
Conclusion: Folic acid-labeled Fe2O3/Au@PAM NPs can serve as dual CT/MRI contrast agents and improve the sensitivities of both modalities for the detection of cancer cells.
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2- E. A. Schellenberger, F. Reynolds, R. Weissleder, and L. Josephson, "Surface-functionalized nanoparticle library yields probes for apoptotic cells." (in eng), ChemBioChem, Vol. 5 (No. 3), pp. 275-9, Mar 5 (2004).
3- F. A. Jaffer and R. Weissleder, "Seeing within: molecular imaging of the cardiovascular system." (in eng), Circ Res, Vol. 94 (No. 4), pp. 433-45, Mar 5 (2004).
4- Nguyen Viet Long, Yong Yang, Toshiharu Teranishi, Cao Thi, Yanqin Cao, and M. Nogami, "Biomedical Applications of Advanced Multifunctional Magnetic Nanoparticles." Journal of Nanoscience and Nanotechnology, Vol. 15pp. 1-17, 12/01 (2015).
5- Rajkumar S and Prabaharan M, "Multi-functional core-shell Fe3O4@Au nanoparticles for cancer diagnosis and therapy." Colloids and Surfaces B: Biointerfaces, Vol. 174pp. 252-59, 2019/02/01/ (2019).
6- Sh Karamipour, M. S. Sadjadi, and N. Farhadyar, "Fabrication and spectroscopic studies of folic acid-conjugated Fe3O4@Au core-shell for targeted drug delivery application." (in eng), Spectrochim Acta A Mol Biomol Spectrosc, Vol. 148pp. 146-55, Sep 5 (2015).
7- Mohammad Madani, Mohammad Mahdi Behzadi, and Sheida Nabavi, "The role of deep learning in advancing breast cancer detection using different imaging modalities: A systematic review." Cancers, Vol. 14 (No. 21), p. 5334, (2022).
8- Md Shafiul Islam, Naima Kaabouch, and Wen Chen Hu, "A survey of medical imaging techniques used for breast cancer detection." in IEEE International Conference on Electro-Information Technology, EIT 2013, (2013): IEEE, pp. 1-5.
9- Jamileh Kadkhoda, Ali Tarighatnia, Mohammad Reza Tohidkia, Nader D Nader, and Ayuob Aghanejad, "Photothermal therapy-mediated autophagy in breast cancer treatment: Progress and trends." Life Sciences, p. 120499, (2022).
10- Shang-Wei Chou, Yu-Hong Shau, Ping-Ching Wu, Yu-Sang Yang, Dar-Bin Shieh, and Chia-Chun Chen, "In vitro and in vivo studies of FePt nanoparticles for dual modal CT/MRI molecular imaging." Journal of the American Chemical Society, Vol. 132 (No. 38), pp. 13270-78, (2010).
11- Ali Tarighatnia et al., "Mucin-16 Targeted Mesoporous Nano-system for Evaluation of Cervical cancer via Dual-modal Computed Tomography and Ultrasonography." New Journal of Chemistry, 10.1039/D1NJ04123A (2021).
12- Anita Ebrahimpour et al., "Detection of glioblastoma multiforme using quantitative molecular magnetic resonance imaging based on 5-aminolevulinic acid: in vitro and in vivo studies." Magnetic Resonance Materials in Physics, Biology and Medicine, pp. 1-13, (2022).
13- Anita Ebrahimpour et al., "Magnetic Metal–Organic Framework Based on Zinc and 5-Aminolevulinic Acid: MR Imaging and Brain Tumor Therapy." Journal of Inorganic and Organometallic Polymers and Materials, Vol. 31pp. 1208-16, (2021).
14- Sara Khademi et al., "Folic acid-cysteamine modified gold nanoparticle as a nanoprobe for targeted computed tomography imaging of cancer cells." Materials Science and Engineering: C, Vol. 89pp. 182-93, (2018).
15- Ali Tarighatnia et al., "Engineering and quantification of bismuth nanoparticles as targeted contrast agent for computed tomography imaging in cellular and animal models." Journal of drug delivery science and technology, Vol. 66p. 102895, (2021).
16- N. Kohler, C. Sun, J. Wang, and M. Zhang, "Methotrexate-modified superparamagnetic nanoparticles and their intracellular uptake into human cancer cells." (in eng), Langmuir, Vol. 21 (No. 19), pp. 8858-64, Sep 13 (2005).
17- Shewaye Lakew Mekuria, Tilahun Ayane Debele, and Hsieh-Chih Tsai, "PAMAM dendrimer based targeted nano-carrier for bio-imaging and therapeutic agents." RSC Advances, 10.1039/C6RA12895E Vol. 6 (No. 68), pp. 63761-72, (2016).
18- Seraj Mohaghegh, Ali Tarighatnia, Yadollah Omidi, Jaleh Barar, Ayuob Aghanejad, and Khosro Adibkia, "Multifunctional magnetic nanoparticles for MRI-guided co-delivery of erlotinib and L-asparaginase to ovarian cancer." Journal of Microencapsulation, Vol. 39 (No. 4), pp. 394-408, (2022).
19- F. M. Sirotnak and B. Tolner, "Carrier-mediated membrane transport of folates in mammalian cells." (in eng), Annu Rev Nutr, Vol. 19pp. 91-122, (1999).
20- Sergio Andrés Torres-Pérez, María del Pilar Ramos-Godínez, and Eva Ramón-Gallegos, "Effect of methotrexate conjugated PAMAM dendrimers on the viability of breast cancer cells." AIP Conference Proceedings, Vol. 2090 (No. 1), p. 050014, 2019/04/10 (2019).
21- Hongdong Cai et al., "Dendrimer-Assisted Formation of Fe3O4/Au Nanocomposite Particles for Targeted Dual Mode CT/MR Imaging of Tumors." Small, https://doi.org/10.1002/smll.201500856 Vol. 11 (No. 35), pp. 4584-93, 2015/09/01 (2015).
22- Limin Guo et al., "Hollow mesoporous carbon spheres—an excellent bilirubin adsorbent." Chemical Communications, 10.1039/B911083F (No. 40), pp. 6071-73, (2009).
23- Heyu Yang et al., "Effects of iron oxide nanoparticles as T2-MRI contrast agents on reproductive system in male mice." Journal of Nanobiotechnology, Vol. 20 (No. 1), p. 98, 2022/03/02 (2022).
24- Chen Bai et al., "Synthesis of Ultrasmall Fe3O4 Nanoparticles as T1–T2 Dual-Modal Magnetic Resonance Imaging Contrast Agents in Rabbit Hepatic Tumors." ACS Applied Nano Materials, Vol. 3 (No. 4), pp. 3585-95, 2020/04/24 (2020).
25- Hongdong Cai et al., "Facile assembly of Fe3O4@Au nanocomposite particles for dual mode magnetic resonance and computed tomography imaging applications." Journal of Materials Chemistry, 10.1039/C2JM16851K Vol. 22 (No. 30), pp. 15110-20, (2012).
| Files | ||
| Issue | Vol 11 No 1 (2024) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/fbt.v11i1.14519 | |
| Keywords | ||
| Breast Cancer Computed Tomography Magnetic Resonance Imaging Targeted Imaging | ||
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How to Cite
1.
Jamshidi N, Tarighatnia A, Fazel Ghaziyani M, Sajadian F, Olad ghaffari M, Nader N. Folic Acid-Conjugated Fe-Au-Based Nanoparticles for Dual Detection of Breast Cancer Cells by Magnetic Resonance Imaging and Computed Tomography. Frontiers Biomed Technol. 2023;11(1):122-129.
