In vivo Biocompatibility of Dental Bonding Agents containing Colloidal Dispersion of Titanium Dioxide

Abstract

Aims (Purpose): To investigate the direct and indirect cytotoxic effects of two universal dental bonding agents incorporated with titanium oxide colloidal dispersion on a human gingival fibroblast cell.

Settings and Design: An in vitro study.

Methods and Material: Two commercial dental bonding agents’ systems, i.e., Ambar universal (FGM, Brasil) and G-Premio Bond Universal (GC, America) were incorporated with 4% by mass of colloidal dispersion containing titanium oxide nanoparticles (TiO2). A cell line human gingival fibroblast cells was prepared from adult rabbits. Two cytotoxic assays were used to investigate the cytotoxic activity of four bonding agent groups on the fibroblast-like cells as following; GA: Ambar Universal (control), GB: Ambar Universal (4% TiO2 incorporated), GC: G-Premio Bond universal (control), and GD: G-Premio Bond (4% TiO2 incorporated). Forty bonding agent samples (5 x 1 mm discs) were prepared from the bonding agent groups and used for MTT assay, and 32 discs were used for the HCS assay.

Statistical analysis used: Statistical analysis was performed using the independent variable t-conducted by the IBM-SPSS software program.

Results: The results from the cytotoxic assays showed a high degree of cytocompatibility for all tested bonding agents. However, the incorporated bonding agent Groups (GII and GIV) showed significantly less cytotoxic effects than their controls. Also, groups GIII and GIV showed significantly higher cytocompatibility than GI and GII.

Conclusions: Incorporation of 4% by mass of colloidal dispersion of TiO2 nanoparticles significantly enhanced the biocompatibility of the tested universal bonding agents in comparison to their control groups.