Injectable Nano-Chitosan /CaCO3 (NCsC) Composite As Noval Pulpotomy Paste in Partially Pulpotomized Rabbit Incisors

Abstract

Purpose: apply of tissue-engineering principles to generate simple and effective dental pulp capping material represent a promising therapeutic option for treating immature and mature tooth with compromised pulp vitality. The aim of the study was to evaluate the pathophysiological response of the pulp to (nano-chitosan/CaCO₃) scaffold as potential novel  capping material on traumatically exposed pulp of rabbit incisors.

Material and methods:24 lower central incisors of New Zealand rabbits were used, subdivided into two group of 12 teeth according to sacrificing time (1 and 4 week), in each group, six teeth were used as control group, the pulp is traumatically exposed and partially amputated left free of capping material, and the other six teeth used as experimental group ,were the amputated pulp capped with (nano-chitosan/CaCO₃) ,the  cavity of both groups sealed with resin modified glass ionomere cement .animal were sacrificed and teeth were collected for histological examination

 Results: At 1 week, both groups showed non-significant difference in inflammatory extent, high significant difference in calcific bridge formation (P=0.002) .and dentin morphology (P=0.002) . at 4 week period  there is non-significant difference in inflammatory response ,high significant difference in dentine bridge formation (P=0.002) but non -significant difference in the dentin morphology (P=0.06) group, (nano-chitosan/CaCO₃) showed faster dentin bridge formation in both period compared to control group

Conclusions: (nano-chitosan/CaCO₃) composite is promising novel pulpotomy material