Antibacterial Activity of Zinc Oxide Nanoparticles Synthesized by Green Eichhornia Crassipes Extract Method

Abstract

Purpose: Zinc oxide nanoparticle (ZnO NPs) production has become more common because of the benefits of the green strategy, which include its ease of use, environmental friendliness, and cheap operating costs. In order to accomplish the goal of green chemistry, the green synthesis process also uses  safe solvents like water and ethanol. ZnO NPs are among the metal oxide-NPs used in antibacterial and bioremediation applications.

Materials and Methods: Energy dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometer (XRD), and field emission scanning electron microscopy (FESEM) were used to analyze the green-produced ZnO.NPs.

Results: ZnO nanoparticles have an average size of 22.89 nm, which is corroborated by FESEM pictures, indicating that the particles are tiny. The excellent purity of ZnO-NPs has been confirmed by EDX data. The antibacterial activity of ZnO NPs was assessed against a few dangerous pathogens. Zinc oxide nanoparticles were shown to have an interesting antibacterial action against both gram-positive and gram-negative bacteria at micromolar concentrations because they exhibit the maximum diameter of inhibition zone at concentrations 100 mg/ml of  S. aureus, E. coli, K. pneumonia, Acintobacret spp, S. fecalis  reaching (27,19,18,17, and 14) mm, respectively while S. pneumonia were resistant.The ZnO NPs recorded at a concentration of 12.5 mg/ml lowest areas of the inhibition zone against the same isolates reaching (16, 11, 11, 12, and 10) mm while S. pneumonia were resistant, respectively, as well.

Conclusion: ZnO-NPs, since they have excellent antibacterial properties, and are biocompatible, they will open up a new line of inquiry for antibacterial agent research because they are stable, nontoxic, and harmless.